2-nitrophenol

Repeated dose toxicity: inhalation

Administrative data

Endpoint:

short-term repeated dose toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Short summary of a study report from Hazleton Lab in 1984 given in a secondary source.

Data source

Reference

Reference 1

Reference Type:

secondary source

Title:

Concise International Chemical Assessment Document 20 - Mononitrophenols

Author:

International Programme on Chemical Safety (IPCS)

Year:

2000

Bibliographic source:

http://www.inchem.org/documents/cicads/cicads/cicad_20.htm (query result from 2011-10-14)

Reference 2

Reference Type:

other: reference in secondary source

Title:

Unnamed

Year:

1984

Materials and methods

Principles of method if other than guideline:

The subacute inhalative toxicity of the test substance was tested in rats by exposure to vapour in a whole-body inhalation system for 6 h/d, 5 d/week and 4 weeks.

GLP compliance:

not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-nitrophenol

EC Number:

201-857-5

EC Name:

2-nitrophenol

Cas Number:

88-75-5

Molecular formula:

C6H5NO3

IUPAC Name:

2-nitrophenol

Details on test material:

- Name of test material (as cited in study report): 2-Nitrophenol

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

not specified

Remarks on MMAD:

MMAD / GSD: not applicable

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE
- System of generating vapour: Melted test substance was used

Analytical verification of doses or concentrations:

not specified

Duration of treatment / exposure:

4 weeks

Frequency of treatment:

6 hours/day, 5 days/week

Doses / concentrations

Remarks:

Doses / Concentrations:
5, 30, and 60 mg/m³
Basis:
no data

No. of animals per sex per dose:

15

Control animals:

other: Yes (not further specified)

Details on study design:

no data

Examinations

Observations and examinations performed and frequency:

CAGE SIDE OBSERVATIONS: Yes

HAEMATOLOGY: Yes
- Time schedule for collection of blood: After 11th exposure
- Parameters checked: Methaemoglobin values

No further details are given.

Sacrifice and pathology:

GROSS PATHOLOGY: Yes
HISTOPATHOLOGY: Yes

Results and discussion

Results of examinations

Details on results:

Except for squamous metaplasia of the epithelium lining the maxillo-turbinates and naso-turbinates in all high-dose animals, the clinical and histopathological examinations gave no consistent exposure-related effects.
The methaemoglobin values determined after the 11th exposure were significantly increased only in low-dose animals (males: 1.0, 2.3, 1.8, and 1.6 %; females: 2.0, 4.1, 2.1, and 1.1 %), but were within control values at the end of the study.

Effect levels

Dose descriptor:

NOAEL

Effect level:

0.06 mg/L air

Based on:

test mat.

Sex:

male/female

Basis for effect level:

other: Squamous metaplasia of the epithelium lining the maxillo-turbinates and naso-turbinates was observed in all animals of the 0.06 mg/l air dose group.

Target system / organ toxicity

Critical effects observed:

not specified