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The short-term toxicity of 1-chloro-2-methylbenzene towards fish was tested following the OECD Guideline 203 yielding a LC50(96 h) of 7.7 mg/L (NITE 2000).

The short-term toxicity of 1 -chloro-2 -methylbenzene towards aquatic invertebrates is characterized by an EC50(48 h) of 0.7 mg/L having Daphnia magna as test species (NITE 2000).

The long-term toxicity of 1 -chloro-2 -methylbenzene towards aquatic invertebrates was investigated following a preliminary guideline proposal of the German Federal Environmental Agency. Daphnia magna was used as test organism yielding a 21-day NOEC of 0.14 mg/L with reproduction as most sensitive endpoint (Kühn, 1988).

The toxicity of 1-chloro-2-methylbenzene towards aquatic algae is characterized by an EC50(72 h) of 7.8 mg/L, and NOEC(72 h) of 2.6 mg/L using the growth rate as endpoint (NITE 2000).

The toxicity of 1 -chloro-2 -methylbenzene towards microorganisms was tested using Pseudomonas putida as test species. An EC3(16 h) of 15 mg/L was observed having the inhibition of cell multiplication inhibition as endpoint (Bringmann, 1976).