Bis(4-chlorophenyl) sulphone

Specific investigations: other studies

Administrative data

Endpoint:

endocrine system modulation

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Well documented study which meets generally accepted scientific standards

Data source

Reference

Reference Type:

publication

Title:

Screening of some anti-androgenic endocrine disruptors using a recombinant cell-based in vitro bioassay

Author:

Roy P, Salminen H, Koskimies P, Simola J, Smeds A, Saukko P & Huhtaniemi IT

Year:

2004

Bibliographic source:

PMID: 15084347, J Steroid Biochem Mol Biol. 88(2):157-66

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Cell-based androgen reporter assay using the Chinese hamster ovarian cell line (CHO K1) in the 96 -well format. The purpose of this assay is a high throughput screening for endocrine disrupting (ED) substances in environmental and biological samples.
CHO cells were cotransfected with plasmids encoding mouse mammary tumour virus-neomycin-luciferase and human androgen receptor (hAR). A stable cell line was established. After selection with neomycin, a highly active clone was obtained which stably expressed both the hAR and the androgen-responsive luciferase reporter.

GLP compliance:

no

Type of method:

in vitro

Test material

Test material information

Constituent 1

Reference substance name:

Bis(4-chlorophenyl) sulphone

EC Number:

201-247-9

EC Name:

Bis(4-chlorophenyl) sulphone

Cas Number:

80-07-9

Molecular formula:

C12H8Cl2O2S

IUPAC Name:

1-chloro-4-(4-chlorobenzenesulfonyl)benzene

Details on test material:

- Name of test material (as cited in study report): 4,4'-Dichlorodiphenyl sulphone

Administration / exposure

Analytical verification of doses or concentrations:

no

Details on analytical verification of doses or concentrations:

The concentrations of the stock solutions of all the test compounds were 1 mmol/L in ethanol. They were then further diluted in the medium, resulting in the final concentration of ethanol in the incubations of 0.01%. Thus the final concentration was 0.01/100 mmol/L = 0.0001 mmol/L = 0.1 µmol/L.

Doses / concentrations

Remarks:

Doses / Concentrations:
0.1 µmol/L = 0.287 ng/L for anti-androgenic effects up to 50 µmol/L
Basis:
nominal conc.

Examinations

Examinations:

stimulation of luciferase activity

Positive control:

R1881 (Metribolone CAS 965-93-5)

Results and discussion

Details on results:

Test result:
- DCDPS incubation to the in vitro asssay resulted in a negative response. Therefore no androgenic or anti-androgenic effects were identified.

Validity of the assay:
- the test system was idendified as specific and functioning, since stimulation of the cells with androgens for 24h resulted in about 15-fold stimulation of luciferase activity. Furthermore potent steroidal and non-steroidal anti-androgens significantly inhibited the androgen-induced transactivation.
- non-androgenic steroids showed weak activity at high concentrations.
- RT-PCR and western blot confirmed proper transcription and translation as well as stable expression of the AR gene in the cells.