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Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: screening tests
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
QSAR prediction: QSAR method cited as reliable in the REACH guidance
Qualifier:
no guideline required
Principles of method if other than guideline:
QSAR-BioWin v 4.10
GLP compliance:
no
Remarks:
not applicable. QSAR model
Oxygen conditions:
other: QSAR
Inoculum or test system:
other: QSAR
Details on inoculum:
QSAR
Parameter:
other: BIOWIN v4.10
Remarks on result:
other: Ready Biodegradability Prediction: YES
Details on results:
--------------------------- BIOWIN v4.10 Results ----------------------------

Biowin1 (Linear Model Prediction) : Biodegrades Fast
Biowin2 (Non-Linear Model Prediction): Biodegrades Fast
Biowin3 (Ultimate Biodegradation Timeframe): Weeks
Biowin4 (Primary Biodegradation Timeframe): Days
Biowin5 (MITI Linear Model Prediction) : Biodegrades Fast
Biowin6 (MITI Non-Linear Model Prediction): Biodegrades Fast
Biowin7 (Anaerobic Model Prediction): Biodegrades Fast
Ready Biodegradability Prediction: YES

IOWIN (v4.10) Program Results:

==============================

SMILES : CCCCCCCCCCCCO

CHEM : Alcohols, C12-14

MOL FOR: C13H28O

MOL WT : 200.37

--------------------------- BIOWIN v4.10 Results ----------------------------

Biowin1 (Linear Model Prediction) : Biodegrades Fast

Biowin2 (Non-Linear Model Prediction): Biodegrades Fast

Biowin3 (Ultimate Biodegradation Timeframe): Weeks

Biowin4 (Primary Biodegradation Timeframe): Days

Biowin5 (MITI Linear Model Prediction) : Biodegrades Fast

Biowin6 (MITI Non-Linear Model Prediction): Biodegrades Fast

Biowin7 (Anaerobic Model Prediction): Biodegrades Fast

Ready Biodegradability Prediction: YES

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin1 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 1 | Linear C4 terminal chain [CCC-CH3] | 0.1084 | 0.1084

Frag | 1 | Aliphatic alcohol [-OH] | 0.1587 | 0.1587

MolWt| * | Molecular Weight Parameter | | -0.0887

Const| * | Equation Constant | | 0.7475

============+============================================+=========+=========

RESULT | Biowin1 (Linear Biodeg Probability) | | 0.9260

============+============================================+=========+=========

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin2 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 1 | Linear C4 terminal chain [CCC-CH3] | 1.8437 | 1.8437

Frag | 1 | Aliphatic alcohol [-OH] | 1.1178 | 1.1178

MolWt| * | Molecular Weight Parameter | | -2.6460

============+============================================+=========+=========

RESULT | Biowin2 (Non-Linear Biodeg Probability) | | 0.9652

============+============================================+=========+=========

A Probability Greater Than or Equal to 0.5 indicates --> Biodegrades Fast

A Probability Less Than 0.5 indicates --> Does NOT Biodegrade Fast

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin3 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 1 | Linear C4 terminal chain [CCC-CH3] | 0.2983 | 0.2983

Frag | 1 | Aliphatic alcohol [-OH] | 0.1600 | 0.1600

MolWt| * | Molecular Weight Parameter | | -0.4118

Const| * | Equation Constant | | 3.1992

============+============================================+=========+=========

RESULT | Biowin3 (Survey Model - Ultimate Biodeg) | | 3.2457

============+============================================+=========+=========

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin4 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 1 | Linear C4 terminal chain [CCC-CH3] | 0.2691 | 0.2691

Frag | 1 | Aliphatic alcohol [-OH] | 0.1294 | 0.1294

MolWt| * | Molecular Weight Parameter | | -0.2688

Const| * | Equation Constant | | 3.8477

============+============================================+=========+=========

RESULT | Biowin4 (Survey Model - Primary Biodeg) | | 3.9774

============+============================================+=========+=========

Result Classification: 5.00 -> hours 4.00 -> days 3.00 -> weeks

(Primary & Ultimate) 2.00 -> months 1.00 -> longer

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin5 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 1 | Aliphatic alcohol [-OH] | 0.1611 | 0.1611

Frag | 1 | Methyl [-CH3] | 0.0004 | 0.0004

Frag | 11 | -CH2- [linear] | 0.0494 | 0.5436

MolWt| * | Molecular Weight Parameter | | -0.5544

Const| * | Equation Constant | | 0.7121

============+============================================+=========+=========

RESULT | Biowin5 (MITI Linear Biodeg Probability) | | 0.8629

============+============================================+=========+=========

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin6 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 1 | Aliphatic alcohol [-OH] | 1.0041 | 1.0041

Frag | 1 | Methyl [-CH3] | 0.0194 | 0.0194

Frag | 11 | -CH2- [linear] | 0.4295 | 4.7244

MolWt| * | Molecular Weight Parameter | | -5.3794

============+============================================+=========+=========

RESULT |Biowin6 (MITI Non-Linear Biodeg Probability)| | 0.9476

============+============================================+=========+=========

A Probability Greater Than or Equal to 0.5 indicates --> Readily Degradable

A Probability Less Than 0.5 indicates --> NOT Readily Degradable

------+-----+--------------------------------------------+---------+---------

TYPE | NUM | Biowin7 FRAGMENT DESCRIPTION | COEFF | VALUE

------+-----+--------------------------------------------+---------+---------

Frag | 1 | Linear C4 terminal chain [CCC-CH3] | -0.3177 | -0.3177

Frag | 1 | Aliphatic alcohol [-OH] | 0.1328 | 0.1328

Frag | 1 | Methyl [-CH3] | -0.0796 | -0.0796

Frag | 11 | -CH2- [linear] | 0.0260 | 0.2859

Const| * | Equation Constant | | 0.8361

============+============================================+=========+=========

RESULT | Biowin7 (Anaerobic Linear Biodeg Prob) | | 0.8574

============+============================================+=========+=========

A Probability Greater Than or Equal to 0.5 indicates --> Biodegrades Fast

A Probability Less Than 0.5 indicates --> Does NOT Biodegrade Fast

Ready Biodegradability Prediction: (YES or NO)

----------------------------------------------

Criteria for the YES or NO prediction: If the Biowin3 (ultimate survey

model) result is "weeks" or faster (i.e. "days", "days to weeks", or

"weeks" AND the Biowin5 (MITI linear model) probability is >= 0.5, then

the prediction is YES (readily biodegradable). If this condition is not

satisfied, the prediction is NO (not readily biodegradable). This method

is based on application of Bayesian analysis to ready biodegradation data

(see Help). Biowin5 and 6 also predict ready biodegradability, but for

degradation in the OECD301C test only; using data from the Chemicals

Evaluation and Research Institute Japan (CERIJ) database.

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
The BIOWIN QSAR predicts that the substance Alcohols, C12-14 will be readily biodegradable.
Executive summary:

The estimated Biodegradetion in Water was measured by calculation from EPI SuiteTM v4.1/BIOWIN v4.10 Program and Ready Biodegradation was predicted. This is Exposure Assessment Tools and Models made from EPA (Environmental Protection Agency).

 

BIOWIN v4.10 Results

Biowin1 (Linear Model Prediction) : Biodegrades Fast

Biowin2 (Non-Linear Model Prediction): Biodegrades Fast

Biowin3 (Ultimate Biodegradation Timeframe): Weeks

Biowin4 (Primary Biodegradation Timeframe): Days

Biowin5 (MITI Linear Model Prediction) : Biodegrades Fast

Biowin6 (MITI Non-Linear Model Prediction): Biodegrades Fast

Biowin7 (Anaerobic Model Prediction): Biodegrades Fast

Ready Biodegradability Prediction: YES

 

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
comparable to guideline study
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
Qualifier:
according to guideline
Guideline:
other: BOD-test for Insoluble Substances (BODIS); ISO 10708 (1997)
Principles of method if other than guideline:
Method:BOD-test for Insoluble Substances (BODIS); ISO 10708 (1997)
Water quality-Evaluation in an aqueous medium of the ultimate aerobic biodegradability of organic compounds - Determination of biochemical oxygen demand in a two-phase closed bottle test.
The test method used is based on OECD test method 301D and the RDA-Blok-Test. Mineral medium was inoculated with activated sludge and stabilized for one week at 18-22 C with continuous stirring. After stabilisation, 200 ml of test medium was filled into 300 ml bottles, aerated until O2 saturation was reached and spiked with test substance by directly weighing into the test vessels.
Vessels were filled 2/3, stoppered and shaken continuously at 18-22 C. Degradation was followed by weekly measurements of BOD using an O2-electrode. Oxygen consumption resulting from biodegradation of the test substance was corrected by oxygen uptake of blank inoculum. Degradation rate was calculated as % BOD/ThOD.


GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
other: effluent from domestic sewage treatment plant (appr.1000 - 100000 cells/ml)
Details on inoculum:
effluent from domestic sewage treatment plant (appr.1000 - 100000 cells/ml)
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
ThOD/L
Reference substance:
other: Sodium acetate
Parameter:
% degradation (test mat. analysis)
Value:
11 - 17
Sampling time:
2 d
Parameter:
% degradation (test mat. analysis)
Value:
14 - 74
Sampling time:
11 d
Parameter:
% degradation (test mat. analysis)
Value:
69 - 85
Sampling time:
14 d
Parameter:
% degradation (test mat. analysis)
Value:
75 - 91
Sampling time:
21 d
Parameter:
% degradation (test mat. analysis)
Value:
79 - 97
Sampling time:
28 d
Details on results:
Degradation: = 79 - 97 % after 28 day(s) , the substance is considered to be readily biodegradable.

Results with reference substance:
Kinetic of control substance:
2 days = 28 - 33%
7 days = 76 - 73%
14 days = 82 - 80%
21 days = 84 -84%
28 days = 87 - 86%

Two concentrations of effluent were tested:

5 ml/l and 50 ml/l. In the results section, the first value cited is for the 5 ml/l concentration and the second set refers to the 50ml/l concentration.The test substance degraded >60% over the test period, meeting the 10 day window criterion, therefore it is considered readily biodegradable..

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Degradation: = 79 - 97 % after 28 day(s) , the substance is considered to be readily biodegradable.
Executive summary:

Degradation: = 79 - 97 % after 28 day(s) , the substance is considered to be readily biodegradable.

.

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
other: published data
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to guideline
Guideline:
ISO Draft (BOD Test for Insoluble Substances)
Principles of method if other than guideline:
Method: two phase closed bottle test
GLP compliance:
not specified
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, adapted
Details on inoculum:
effluent from predominantly domestic sewage treatment plant
Duration of test (contact time):
28 d
Initial conc.:
50 mg/L
Based on:
COD
Reference substance:
not specified
Parameter:
% degradation (test mat. analysis)
Value:
56 - 62
Sampling time:
28 d
Details on results:
Degradation: = 56 - 62 % after 28 day(s) , the substance is considered to be readily biodegradable.

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Degradation: = 56 - 62 % after 28 day(s) , the substance is considered to be readily biodegradable.
Executive summary:

Degradation: = 56 - 62 % after 28 day(s) , the substance is considered to be readily biodegradable.

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
other: published data
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
comparable to guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
Principles of method if other than guideline:
Method: EEC-Directive 92/69/EEC Annex V, Part C: Methods for the Determination of ecotoxicity.
C.4. Biodegradation: determination of the 'ready biodegradability' C.4-E.
This method corresponds to OECD test method 301D.


GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
other: effluent of predominantly domestic sewage treatment plant
Details on inoculum:
INOCULUM/TEST ORGANISM
Sampling site: Plant Hochdahl, Germany
INITIAL TEST SUBSTANCE CONCENTRATION: 2 and 5 mg/l
METHOD OF PREPARATION OF TEST SOLUTION: an inert emulsifier (nonylphenol ethoxylated propoxylated, NP+9.5 EO+5PO) was used to disperse the test substance. Concentration of emulsifier not reported.

Duration of test (contact time):
28 d
Initial conc.:
2 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- Composition of medium: not reported

- Additional substrate: none

- Solubilising agent (type and concentration if used): an inert emulsifier (nonylphenol ethoxylated propoxylated, NP+9.5 EO+5PO) was used to disperse the test substance. Concentration of emulsifier not reported.

- Test temperature: 20 +/- 1C

- pH: not reported

- Aeration of dilution water: not reported

- Suspended solids concentration: not reported

- Nitrate/nitrite measurement: Yes

TEST SYSTEM
- Initial test substance concentration: 2 and 5 mg/l

CONTROL AND BLANK SYSTEM
Controls: mineral medium/ mineral medium with inoculum/mineral medium with inoculum and emulsifier. Degradation rate of test substance was corrected by oxygen uptake of blank inoculum and emulsifier control.
Reference substance:
benzoic acid, sodium salt
Parameter:
% degradation (DOC removal)
Value:
54
Sampling time:
7 d
Parameter:
% degradation (DOC removal)
Value:
68
Sampling time:
14 d
Parameter:
% degradation (DOC removal)
Value:
80
Sampling time:
21 d
Parameter:
% degradation (DOC removal)
Value:
79
Sampling time:
28 d
Details on results:
Degradation: = 79 % after 28 day(s) , the substance is considered to be readily biodegradable.
The test substance (2 mg/l) attained >60% degradation during the 14 day window.
The values reported in the results section are for the 2 mg/l concentration. The 5 mg/l concentration of test substance had insufficient residual dissolved oxygen content after 21 days.
Results with reference substance:
The reference substance attained a biodegradation value of 88% in 28 days.

Table: Degradation kinetics

Type of suspension

% degradation at sampling time (days)

7

14

21

28

 

 

 

 

 

Test sample (2mg/l)

54

68

80

79

 

 

 

 

 

Reference substance

76

78

76

88

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Degradation: = 79 % after 28 day(s) , the substance is considered to be readily biodegradable.Dodecan-1-ol (C12) is supporting substance for Alcohols,C12-C14 and the main component.
The test substance (2 mg/l) attained >60% degradation during the 14 day window.
The values reported in the results section are for the 2 mg/l concentration. The 5 mg/l concentration of test substance had insufficient residual dissolved oxygen content after 21 days.

Executive summary:

Degradation: = 79 % after 28 day(s) , the substance is considered to be readily biodegradable.

The test substance (2 mg/l) attained >60% degradation during the 14 day window.

The values reported in the results section are for the 2 mg/l concentration. The 5 mg/l concentration of test substance had insufficient residual dissolved oxygen content after 21 days.

.

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
other: published data
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to guideline
Guideline:
other: ISO 10708 (BODIS)
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
Principles of method if other than guideline:
Method: The test method used is based on OECD test method 301D and the RDA-Blok-Test. It is especially suitable for poorly water-soluble compounds. The test medium is inoculated and the test chemical added. The test vessels are then closed and shaken continuously.
Weekly measurements of the BOD from the aqueous phase are taken.

GLP compliance:
not specified
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
Concentration of activated sludge: 30 mg dry matter/l
Test volume: 200 ml
Temperature: 20-25 C
pH: not reported
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
COD
Details on study design:
Total oxygen uptake in flasks is calculated from blank-corrected decrease in measured dissolved oxygen concentration divided by saturation value at normal conditions and multiplied with total oxygen content originally present in liquid and gas phase.
Reference substance:
other: sodium acetate trihydrate
Parameter:
% degradation (test mat. analysis)
Value:
72
Sampling time:
7 d
Parameter:
% degradation (test mat. analysis)
Value:
89
Sampling time:
14 d
Parameter:
% degradation (test mat. analysis)
Value:
93
Sampling time:
21 d
Parameter:
% degradation (test mat. analysis)
Value:
100
Sampling time:
28 d
Details on results:
Degradation: = 100 % after 28 day(s) , the substance is considered to be readily biodegradable.
The substance degraded >60% in the 10 day window.
Results with reference substance:
The reference substance attained a biodegradation value of 88% in 28 days.
Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Degradation: = 100 % after 28 day(s) , the substance is considered to be readily biodegradable.
The substance degraded >60% in the 10 day window. .Dodecan-1-ol (C12) is supporting substance for Alcohols,C12-C14 and the main component.
Executive summary:

Degradation: = 100 % after 28 day(s) , the substance is considered to be readily biodegradable.

The substance degraded >60% in the 10 day window. .Dodecan-1-ol (C12) is supporting substance for Alcohols,C12-C14 and the main component.

.

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
other: published data
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Principles of method if other than guideline:
This test method corresponds to OECD 301B
The following validity criteria were met:
(1) the blanks were valid for this test, the maximum milligrams of carbon dioxide were well within the 40 mg/l range,
(2) both sodium acetate samples obeyed the 10-60% rule, no days were required for the bacterial population to acclimate to the sodium acetate,
(3) Parallel assays did not differ by more than 20%.



GLP compliance:
not specified
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Duration of test (contact time):
28 d
Initial conc.:
20 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
TEST CONDITIONS
- Composition of medium:

- Continuous darkness: yes

TEST SYSTEM
- Culturing apparatus: Flasks

- Number of culture flasks/concentration: 6 flasks per sample, 3L of solution per flask

- Method used to create aerobic conditions: Continuous aeration or mixing

SAMPLING
- Sampling frequency: Continuous
Reference substance:
other: Sodium acetate
Parameter:
% degradation (CO2 evolution)
Value:
16
Sampling time:
4 d
Parameter:
% degradation (CO2 evolution)
Value:
44
Sampling time:
8 d
Parameter:
% degradation (CO2 evolution)
Value:
60
Sampling time:
14 d
Parameter:
% degradation (CO2 evolution)
Value:
71
Sampling time:
28 d
Parameter:
% degradation (CO2 evolution)
Value:
73
Sampling time:
46 d
Details on results:
Degradation: = 71 % after 28 day(s) , the substance is considered to be readily biodegradable.
The substance degraded >60% in the 14 day window. .Dodecan-1-ol (C12) is supporting substance for Alcohols,C12-C14 and the main component. The reference substance Sodium acetate degraded by 78% after 28 days.
Results with reference substance:
Reference substance = 78% in 28 days
Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Degradation: = 71 % after 28 day(s) , the substance is considered to be readily biodegradable.
The substance degraded >60% in the 14 day window. .Dodecan-1-ol (C12) is supporting substance for Alcohols,C12-C14 and the main component. The reference substance Sodium acetate degraded by 78% after 28 days.
Executive summary:

Degradation: = 71 % after 28 day(s) , the substance is considered to be readily biodegradable.

The substance degraded >60% in the 14 day window. .Dodecan-1-ol (C12) is supporting substance for Alcohols,C12-C14 and the main component. The reference substance Sodium acetate degraded by 78% after 28 days.

Description of key information

Biodegradation in water:screening tests 
Data of following studies are demonstrating rapid biodegradation Alcohols, C12-14 under aerobic conditions.

Henkel KGaA. , 1997 conducted BOD-test for Insoluble Substances (BODIS); ISO 10708 for Biodegradation of Alcohols, C12-14.
The test method used is based on OECD test method 301D and the RDA-Blok-Test. Mineral medium was inoculated with activated sludge and stabilized for one week at 18-22 C with continuous stirring. After stabilisation, 200 ml of test medium was filled into 300 ml bottles, aerated until O2 saturation was reached and spiked with test substance by directly weighing into the test vessels.
Degradation was 79 - 97 % after 28 day(s), the substance is considered to be readily biodegradable
The BIOWIN QSAR predicts that the substance Alcohols, C12-14 will be readily biodegradable.
Richterich , 1993 conducted OECD 301D test for Biodegradation of Alcohols, C12 (1-Dodecanol). Degradation was 79 % after 28 day(s) , the substance is considered to be readily biodegradable. Dodecan-1-ol (C12) is supporting substance for Alcohols, C12-C14 and the main component. The test substance (2 mg/l) attained >60% degradation during the 14 day window.

Henkel KGaA. , 1992 conducted OECD test method 301D and BOD-test for Insoluble Substances (BODIS); ISO 10708 for Biodegradation of Dodecan-1-ol (C12. Degradation was 100 % after 28 day(s) , the substance is considered to be readily biodegradable.
The substance degraded >60% in the 10 day window. .Dodecan-1-ol (C12) is supporting substance for Alcohols, C12-C14 and the main component.
Morris, P.A., Filler, P.A., and Nielsen, A.M. (1991) conducted OECD 301B test and Degradationwas 71 % after 28 day(s) , the substance is considered to be readily biodegradable. The substance degraded >60% in the 14 day window. .Dodecan-1-ol (C12) is supporting substance for Alcohols,C12-C14 and the main component.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

Biodegradation in water:screening tests

 

Data of following studies are demonstrating rapid biodegradation Alcohols, C12-14 under aerobic conditions.

 

 Henkel KGaA. , 1997 conducted BOD-test for Insoluble Substances (BODIS); ISO 10708 for Biodegradation of Alcohols, C12-14.

The test method used is based on OECD test method 301D and the RDA-Blok-Test. Mineral medium was inoculated with activated sludge and stabilized for one week at 18-22 C with continuous stirring. After stabilisation, 200 ml of test medium was filled into 300 ml bottles, aerated until O2 saturation was reached and spiked with test substance by directly weighing into the test vessels.

Degradation was 79 - 97 % after 28 day(s), the substance is considered to be readily biodegradable

 

The BIOWIN QSAR predicts that the substance Alcohols, C12-14 will be readily biodegradable.

 

Richterich , 1993 conducted OECD 301D test for Biodegradation of Alcohols, C12 (1-Dodecanol). Degradation was 79 % after 28 day(s) , the substance is considered to be readily biodegradable. Dodecan-1-ol (C12) is supporting substance for Alcohols, C12-C14 and the main component. The test substance (2 mg/l) attained >60% degradation during the 14 day window.

 

Henkel KGaA. , 1992 conducted OECD test method 301D and BOD-test for Insoluble Substances (BODIS); ISO 10708 for Biodegradation of Dodecan-1-ol (C12. Degradation was 100 % after 28 day(s) , the substance is considered to be readily biodegradable.

The substance degraded >60% in the 10 day window. .Dodecan-1-ol (C12) is supporting substance for Alcohols, C12-C14 and the main component.

Morris, P.A., Filler, P.A., and Nielsen, A.M. (1991) conducted OECD 301B test and Degradationwas 71 % after 28 day(s) , the substance is considered to be readily biodegradable. The substance degraded >60% in the 14 day window. .Dodecan-1-ol (C12) is supporting substance for Alcohols,C12-C14 and the main component. The reference substance Sodium acetate degraded by 78% after 28 days.

 

 

 

Table     Summary of Biodegradation in water:screening tests

 

Type/ Method

Test material

Degradation

Duration

References

 

Aerobic/

OECD 301D/(BODIS)

Alcohols, C12-14

79-97% in 28 days at 100 mg/L

28 days

Henkel KGaA. , 1997

 

Aerobic/

OECD 301D

Alcohols, C12 (1-Dodecanol).

 

79%

 

28 days

 

Richterich , 1993

 

Aerobic/

OECD 301D/(BODIS

Alcohols, C12 (1-Dodecanol).

 

100 %

28 days

Henkel KGaA. , 1992

 

Aerobic/

OECD 301D

Alcohols, C12 (1-Dodecanol).

 

71 %

28 days

Morris, P.A., Filler, P.A., and Nielsen, A.M. (1991)