Registration Dossier

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Link to relevant study record(s)

Referenceopen allclose all

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to guideline
Guideline:
other: OECD Oxygen Consumption Test
Principles of method if other than guideline:
Method: German standard methods for the examination of water, waste water and sludge; bioassays (group L); determination of the inhibitory effect of waste water on the oxygen consumption of pseudomonas putida (L 27); DIN 38412 part 27.
The oxygen consumption rate of a bacterial suspension fed with glucose as nutrient base is measured after a contact time of 30 minutes. The oxygen consumption rate of the same bacterial suspension in the presence of various concentrations of a test substance under otherwise identicl conditions is also measured. The inhibitory effect of the test substance at a particular concentration is expressed as a percentage of the mean oxygen consumption rates of the controls. An EC0 value can be determined from these measurements.
GLP compliance:
not specified
Analytical monitoring:
not specified
Vehicle:
no
Test organisms (species):
Pseudomonas putida
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
30 min
Nominal and measured concentrations:
10000 mg/l was highest concentration tested.
Details on test conditions:
The oxygen consumption rate of a bacterial suspension fed with glucose as nutrient base is measured after a contact time of 30 minutes. The oxygen consumption rate of the same bacterial suspension in the presence of various concentrations of a test substance under otherwise identicl conditions is also measured. The inhibitory effect of the test substance at a particular concentration is expressed as a percentage of the mean oxygen consumption rates of the controls. An EC0 value can be determined from these measurements.
Reference substance (positive control):
not specified
Duration:
30 min
Dose descriptor:
EC0
Effect conc.:
> 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks on result:
other: non-toxic at the limit of solubility
Details on results:
Acute toxicity to Microorganism (Pseudomonas putida): 30min-EC0 is >10000 mg/L.
The water solubility of the compound is approximately 4.0 mg/l, therefore the EC50 was not achieved at the solubility limit.
Validity criteria fulfilled:
yes
Conclusions:
Acute toxicity to Microorganism (Pseudomonas putida): 30min-EC0 is >10000 mg/L.
The water solubility of the compound is approximately 4.0 mg/l, therefore the EC50 was not achieved at the solubility limit.
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Principles of method if other than guideline:
Method: DIN 38412, Teil 27 (Bacterial oxygen consumption test) method for the examination of water, waste water and sludge; bioassays (group L); determination of the inhibitory effect of waste water on the oxygen consumption of pseudomonas putida (L 27); DIN 38412 part 27.
The oxygen consumption rate of a bacterial suspension fed with glucose as nutrient base is measured after a contact time of 30 minutes. The oxygen consumption rate of the same bacterial suspension in the presence of various concentrations of a test substance under otherwise identicl conditions is also measured. The inhibitory effect of the test substance at a particular concentration is expressed as a percentage of the mean oxygen consumption rates of the controls. An EC0 value can be determined from these measurements.
GLP compliance:
not specified
Analytical monitoring:
not specified
Vehicle:
no
Test organisms (species):
Pseudomonas putida
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
30 min
Nominal and measured concentrations:
10000 mg/l was highest concentration tested.
Details on test conditions:
The oxygen consumption rate of a bacterial suspension fed with glucose as nutrient base is measured after a contact time of 30 minutes. The oxygen consumption rate of the same bacterial suspension in the presence of various concentrations of a test substance under otherwise identicl conditions is also measured. The inhibitory effect of the test substance at a particular concentration is expressed as a percentage of the mean oxygen consumption rates of the controls. An EC0 value can be determined from these measurements.
Reference substance (positive control):
not specified
Duration:
30 min
Dose descriptor:
EC0
Effect conc.:
10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks on result:
other: non-toxic at the limit of solubility
Details on results:
Acute toxicity to Microorganism (Pseudomonas putida): 30min-EC0 is 10000 mg/L.
The water solubility of the compound is approximately 4.0 mg/l, therefore the EC50 was not achieved at the solubility limit.
Validity criteria fulfilled:
yes
Conclusions:
Acute toxicity to Microorganism (Pseudomonas putida): 30min-EC0 is 10000 mg/L.
The water solubility of the compound is approximately 4.0 mg/l, therefore the EC50 was not achieved at the solubility limit.
Endpoint:
activated sludge respiration inhibition testing
Type of information:
other: published data
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Version / remarks:
OECD Oxygen Consumption Test
Principles of method if other than guideline:
Method: DIN 38412, Teil 27 (Bacterial oxygen consumption test) method for the examination of water, waste water and sludge; bioassays (group L); determination of the inhibitory effect of waste water on the oxygen consumption of pseudomonas putida (L 27); DIN 38412 part 27.
The oxygen consumption rate of a bacterial suspension fed with glucose as nutrient base is measured after a contact time of 30 minutes. The oxygen consumption rate of the same bacterial suspension in the presence of various concentrations of a test substance under otherwise identicl conditions is also measured. The inhibitory effect of the test substance at a particular concentration is expressed as a percentage of the mean oxygen consumption rates of the controls. An EC0 value can be determined from these measurements.

GLP compliance:
yes
Analytical monitoring:
not specified
Vehicle:
no
Test organisms (species):
Pseudomonas putida
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
30 min
Details on test conditions:
The oxygen consumption rate of a bacterial suspension fed glucose as nutrient base is measured after a contact time of 30 minutes. The oxygen consumption rate of the same bacterial suspension in the presence of various concentrations of a test substance under otherwise identical conditions is also measured.
Reference substance (positive control):
not specified
Duration:
30 min
Dose descriptor:
EC0
Effect conc.:
> 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks on result:
other: non-toxic at the limit of solubility,
Details on results:
Acute toxicity to Microorganism (Pseudomonas putida): 30min-EC0 is >10000 mg/L.
Validity criteria fulfilled:
yes
Conclusions:
Acute toxicity to Microorganism (Pseudomonas putida): 30min-EC0 is >10000 mg/L.
The water solubility of the compound is approximately 4.0 mg/l, therefore the EC50 was not achieved at the solubility limit.

Description of key information

Acute toxicity to Microorganism (Pseudomonas putida): 30min-EC0 is >10000 mg/L.
The water solubility of the compound is approximately 4.0 mg/l, therefore the EC50 was not achieved at the solubility limit.

Key value for chemical safety assessment

EC50 for microorganisms:
50 000 mg/L
EC10 or NOEC for microorganisms:
10 000 mg/L

Additional information

Acute toxicity to Microorganism (Pseudomonas putida): 30min-EC0 is >10000 mg/L. The water solubility of the compound is approximately 4.0 mg/l, therefore the EC50 was not achieved at the solubility limit.