Alcohols, C12-14

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

other: OECD Oxygen Consumption Test

Principles of method if other than guideline:

Method: German standard methods for the examination of water, waste water and sludge; bioassays (group L); determination of the inhibitory effect of waste water on the oxygen consumption of pseudomonas putida (L 27); DIN 38412 part 27.
The oxygen consumption rate of a bacterial suspension fed with glucose as nutrient base is measured after a contact time of 30 minutes. The oxygen consumption rate of the same bacterial suspension in the presence of various concentrations of a test substance under otherwise identicl conditions is also measured. The inhibitory effect of the test substance at a particular concentration is expressed as a percentage of the mean oxygen consumption rates of the controls. An EC0 value can be determined from these measurements.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

no

Test organisms (species):

Pseudomonas putida

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Nominal and measured concentrations:

10000 mg/l was highest concentration tested.

Details on test conditions:

The oxygen consumption rate of a bacterial suspension fed with glucose as nutrient base is measured after a contact time of 30 minutes. The oxygen consumption rate of the same bacterial suspension in the presence of various concentrations of a test substance under otherwise identicl conditions is also measured. The inhibitory effect of the test substance at a particular concentration is expressed as a percentage of the mean oxygen consumption rates of the controls. An EC0 value can be determined from these measurements.

Reference substance (positive control):

not specified

Duration:

30 min

Dose descriptor:

EC0

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks on result:

other: non-toxic at the limit of solubility

Details on results:

Acute toxicity to Microorganism (Pseudomonas putida): 30min-EC0 is >10000 mg/L.
The water solubility of the compound is approximately 4.0 mg/l, therefore the EC50 was not achieved at the solubility limit.

Validity criteria fulfilled:

yes

Conclusions:

Acute toxicity to Microorganism (Pseudomonas putida): 30min-EC0 is >10000 mg/L.
The water solubility of the compound is approximately 4.0 mg/l, therefore the EC50 was not achieved at the solubility limit.

Reference 2

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Principles of method if other than guideline:

Method: DIN 38412, Teil 27 (Bacterial oxygen consumption test) method for the examination of water, waste water and sludge; bioassays (group L); determination of the inhibitory effect of waste water on the oxygen consumption of pseudomonas putida (L 27); DIN 38412 part 27.
The oxygen consumption rate of a bacterial suspension fed with glucose as nutrient base is measured after a contact time of 30 minutes. The oxygen consumption rate of the same bacterial suspension in the presence of various concentrations of a test substance under otherwise identicl conditions is also measured. The inhibitory effect of the test substance at a particular concentration is expressed as a percentage of the mean oxygen consumption rates of the controls. An EC0 value can be determined from these measurements.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

no

Test organisms (species):

Pseudomonas putida

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Nominal and measured concentrations:

10000 mg/l was highest concentration tested.

Details on test conditions:

The oxygen consumption rate of a bacterial suspension fed with glucose as nutrient base is measured after a contact time of 30 minutes. The oxygen consumption rate of the same bacterial suspension in the presence of various concentrations of a test substance under otherwise identicl conditions is also measured. The inhibitory effect of the test substance at a particular concentration is expressed as a percentage of the mean oxygen consumption rates of the controls. An EC0 value can be determined from these measurements.

Reference substance (positive control):

not specified

Duration:

30 min

Dose descriptor:

EC0

Effect conc.:

10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks on result:

other: non-toxic at the limit of solubility

Details on results:

Acute toxicity to Microorganism (Pseudomonas putida): 30min-EC0 is 10000 mg/L.
The water solubility of the compound is approximately 4.0 mg/l, therefore the EC50 was not achieved at the solubility limit.

Validity criteria fulfilled:

yes

Conclusions:

Acute toxicity to Microorganism (Pseudomonas putida): 30min-EC0 is 10000 mg/L.
The water solubility of the compound is approximately 4.0 mg/l, therefore the EC50 was not achieved at the solubility limit.

Reference 3

Endpoint:

activated sludge respiration inhibition testing

Type of information:

other: published data

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Version / remarks:

OECD Oxygen Consumption Test

Principles of method if other than guideline:

Method: DIN 38412, Teil 27 (Bacterial oxygen consumption test) method for the examination of water, waste water and sludge; bioassays (group L); determination of the inhibitory effect of waste water on the oxygen consumption of pseudomonas putida (L 27); DIN 38412 part 27.
The oxygen consumption rate of a bacterial suspension fed with glucose as nutrient base is measured after a contact time of 30 minutes. The oxygen consumption rate of the same bacterial suspension in the presence of various concentrations of a test substance under otherwise identicl conditions is also measured. The inhibitory effect of the test substance at a particular concentration is expressed as a percentage of the mean oxygen consumption rates of the controls. An EC0 value can be determined from these measurements.

GLP compliance:

yes

Analytical monitoring:

not specified

Vehicle:

no

Test organisms (species):

Pseudomonas putida

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Details on test conditions:

The oxygen consumption rate of a bacterial suspension fed glucose as nutrient base is measured after a contact time of 30 minutes. The oxygen consumption rate of the same bacterial suspension in the presence of various concentrations of a test substance under otherwise identical conditions is also measured.

Reference substance (positive control):

not specified

Duration:

30 min

Dose descriptor:

EC0

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks on result:

other: non-toxic at the limit of solubility,

Details on results:

Acute toxicity to Microorganism (Pseudomonas putida): 30min-EC0 is >10000 mg/L.

Validity criteria fulfilled:

yes

Conclusions:

Acute toxicity to Microorganism (Pseudomonas putida): 30min-EC0 is >10000 mg/L.
The water solubility of the compound is approximately 4.0 mg/l, therefore the EC50 was not achieved at the solubility limit.

Description of key information

Acute toxicity to Microorganism (Pseudomonas putida): 30min-EC0 is >10000 mg/L.
The water solubility of the compound is approximately 4.0 mg/l, therefore the EC50 was not achieved at the solubility limit.

Key value for chemical safety assessment

EC50 for microorganisms:

50 000 mg/L

EC10 or NOEC for microorganisms:

10 000 mg/L

Additional information

Acute toxicity to Microorganism (Pseudomonas putida): 30min-EC0 is >10000 mg/L. The water solubility of the compound is approximately 4.0 mg/l, therefore the EC50 was not achieved at the solubility limit.