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EC number: 204-340-2 | CAS number: 119-64-2
Adsorption / desorption
Administrative data
Link to relevant study record(s)
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010-05-04 - 2010-05-25
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
- Version / remarks:
- (2001)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
- Version / remarks:
- (2008)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- HPLC estimation method
- Media:
- soil/sewage sludge
- Radiolabelling:
- no
- Test temperature:
- 23-24ºC
- Details on study design: HPLC method:
- EQUIPMENT
- Apparatus: HPLC System HP 1100 (Agilent) with refractometer HP 1100 (Agilent)
- Type, material and dimension of analytical (guard) column: Zorbax CN, 250 x 4 mm, 5 µm
- Detection system: refractive index
MOBILE PHASES
- Type: methanol / water (purified) 55/45 (v/v)
- Experiments with additives carried out on separate columns: no
- Solutes for dissolving test and reference substances: Solvent 1: methanol / water (purified) 55/45 (v/v); Solvent 2: methanol
DETERMINATION OF DEAD TIME
- Method: by inert substances which are not retained by the column
REFERENCE SUBSTANCES
- Identity: Urea (inert marker),Atrazine, Monuron, Triapenthenol, Linuron, Naphthalene, Fenthion, Trifluralin
DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 49.10 mg /25 mL solvent
- Quantity of reference substances:
Urea 28.37 mg / 25 ml eluent
Atrazine 21.14 mg / 25 ml solvent 1
Monuron 21.42 mg / 25 ml solvent 1
Triapenthenol 21.52 mg / 25 ml solvent 1
Linuron 20.29 mg / 25 ml solvent 1
Naphthalene 25.42 mg / 25 ml solvent 2
Fenthion 34.30 mg / 25 ml solvent 2
Trifluralin 21.80 mg / 25 ml solvent 2
- Intervals of calibration: -
REPETITIONS
- Number of determinations: 2
EVALUATION
- Calculation of capacity factors k':
Atrazine 0.675
Monuron 0.760
Triapenthenol 1.411
Linuron 1.793
Naphthalene 1.667
Fenthion 3.858
Trifluralin 7.695
Test substance1.805
- Calculation of retention times :
Urea 2.46 min.
Atrazine 4.12 min.
Monuron 4.33 min.
Triapenthenol 5.93 min.
Linuron 6.87 min.
Naphthalene 6.56 min.
Fenthion 11.95 min.
Trifluralin 21.39 min.
Test substance 7.01 min.
- Determination of the log Koc value:
Atrazine 1.81
Monuron 1.99
Triapenthenol 2.37
Linuron 2.59
Naphthalene 2.75
Fenthion 3.31
Trifluralin 3.94
Test substance 2.7 - Type:
- Koc
- Value:
- 501
- Temp.:
- 23 °C
- Remarks on result:
- other: HPLC method
- Details on results (HPLC method):
- - Retention times of reference substances used for calibration:
Urea 2.46
Atrazine 4.12
Monuron 4.33
Triapenthenol 5.93
Linuron 6.87
Naphthalene 6.56
Fenthion 11.95
Trifluralin 21.39
- Details of fitted regression line (log k' vs. log Koc): y =1.9767 * x + 2.1695; r2= 0.9939
- Average retention data for test substance: 7.01 minutes - Validity criteria fulfilled:
- yes
- Conclusions:
- The adsorption coefficient on soil (KOC) of the test item 1,2,3,4-tetrahydronaphthalene was determined according to OECD guideline no. 121 [2001] and EU test method C.19 [2008]. The adsorption coefficient on soil (KOC) for this substance was calculated to be KOC = 501 mL/g (log KOC = 2.7; 23-24 ºC).
- Executive summary:
The adsorption coefficient on soil (KOC) of the test item 1,2,3,4-tetrahydronaphthalene was determined according to OECD guideline no. 121 [2001] and EU test method C.19 [2008]. According to this method the test substance is passed through an HPLC column.The dual composition of the stationary phase having polar and non-polar sites allows for interaction of polar and non-polar groups in a similar way as is the case for organic matter in soil or sewage sludge matrices.This enables the relationship between the retention time on the column and the adsorption coefficient on organic matter to be established through the capacity factor K.
The adsorption coefficient on soil (KOC) for this substance was obtained to be KOC = 501 (log KOC = 2.7; 23 -24 ºC) .
The correlation betwen log K and log Koc was good with an r2= 0.9939.
Reference
Description of key information
The adsorption coefficient on soil (KOC) of 1,2,3,4-tetrahydronaphthalene was determined according to OECD guideline no. 121 [2001] and EU test method C.19 [2008]. The resulting KOC was 501 mL/g ( 23-24 ºC).
Key value for chemical safety assessment
- Koc at 20 °C:
- 501
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