3-dimethylaminopropiononitrile

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions. The strains E.Coli WP2 or TA102 are missing. However the study is still suficient for evaluation of nutagenicity in bacterial testsystems

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

yes

Remarks:

E.Coli WP2 or TA102 are missing

GLP compliance:

no

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

3-dimethylaminopropiononitrile

EC Number:

217-090-4

EC Name:

3-dimethylaminopropiononitrile

Cas Number:

1738-25-6

Molecular formula:

C5H10N2

IUPAC Name:

3-(dimethylamino)propanenitrile

Details on test material:

TS-Freetext:
3-Dimethylaminopropionitril
- Name of test material (as cited in study report): Dimethylaminopropionitril
- Analytical purity: >=98%
- Storage condition of test material: Room temperature

Method

Target gene:

his-

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Metabolic activation system:

S-9 mix from Aroclor 1254 induced rat liver

Test concentrations with justification for top dose:

20 µg - 5000 µg/plate

Vehicle / solvent:

Aqua dest.

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

Positive controls:

yes

Positive control substance:

other: see free text

Details on test system and experimental conditions:

Experiment 1: Standard plate test
Test tubes containing 2 ml soft agar kept in a water bath at 45°C, and remaining
components added in the following order:
0.1 ml test solution or vehicle
0.1 ml bacterial suspension
0.5 ml S-9 mix (in tests with metabolic activation) or 0.5 ml phosphate buffer (in tests
without metabolic activation).
After mixing, the samples are poured onto Vogel-Bonner agar plates.

Experiment 2: Preincubation assay
0.1 ml test solution or vehicle, 0.1 ml bacterial suspension and 0.5 ml S-9 mix are
incubated at 37°C for the duration of 20 minutes. Subsequently, 2 ml of soft agar is added
and, after mixing, the samples are poured onto the Vogel-Bonner agar plates.

Experiment1&2
In each experiment 3 test plates per dose or per control used; after incubation at 37°C for
48 hours in the dark, the bacterial colonies (his+ revertants) are counted.
Positive control:
with metabolic activation: 10 μg/plate 2-aminoanthracene for each strain;
without metabolic activation: 5 μg/plate N-methyl-N'-nitro-N-nitrosoguanidine for TA 100 and TA 1535, 10 μg/plate 4-nitro-o-phenylendiamine
for TA 98, 100 μg/plate 9-aminoacridine chloride monohydrate for TA 1537; all substances were dissolved in DMSO.
The titer was determined and in regularly intervals the strain characteristics were checked. Sterility control was performed.

Evaluation criteria:

Positive results
- doubling of the spontaneous mutation rate (control)
- dose-response relationship
- reproducibility of the result

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity nor precipitates, but tested up to recommended limit concentrations

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Additional information on results:

Complete solubility of the test substance in aqua dest.

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'. Remarks: Salmonella typhimurium TA 98 TA 100 TA 1535 TA 1537