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EC number: 247-852-1 | CAS number: 26628-22-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
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- Auto flammability
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- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
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- Endpoint summary
- Stability
- Biodegradation
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- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
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- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
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- Additional toxicological data

Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008-05-16 to 2009-12-02
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.1300 (Acute inhalation toxicity)
- Version / remarks:
- 1998
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- traditional method
- Limit test:
- no
Test material
- Reference substance name:
- Sodium azide
- EC Number:
- 247-852-1
- EC Name:
- Sodium azide
- Cas Number:
- 26628-22-8
- Molecular formula:
- N3Na
- IUPAC Name:
- sodium azide
Constituent 1
- Specific details on test material used for the study:
- - Lot: #C-07A005 and #C-08V701
- Composition: >95% pure
- Physical appearance: White solid, similar in appearance to typical table salt
- pH: 9+
- Solubility: Soluble in water
- Stability: Test substance was expected to be stable for the duration of testing
- Expiration Date: Not Applicable
- Treatment of the test material prior testing: Prior to aerosolization, the test substance was ground in a ball mill for 24 hours and then further ground in a coffee mill (Cuisinart, Model #DC0-20) until it passed through a 425 micrometer sieve (USA standard).
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Remarks:
- albino
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Ace Animals, Inc., Boyertown, PA
- Females (if applicable) nulliparous and non-pregnant: Yes
- Age at study initiation: Young adult (9-10 weeks) /
- Weight at study initiation: males 280-338 grams and females 190-236
- Housing: The animals were singly housed in suspended stainless steel caging with mesh floors. Litter paper was placed beneath the cage and was changed at least three times per week.
- Diet: Purina Rodent Chow #5012
- Water (e.g. ad libitum): Yes
- Acclimation period: 14-18 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21-24°C
- Humidity (%): 66-79%
- Photoperiod (hrs dark / hrs light): 12 / 12
Administration / exposure
- Route of administration:
- inhalation: dust
- Type of inhalation exposure:
- nose only
- Vehicle:
- air
- Mass median aerodynamic diameter (MMAD):
- > 3.5 - < 3.7 µm
- Geometric standard deviation (GSD):
- > 1.88 - < 2.16
- Remark on MMAD/GSD:
- The aerodynamic mass median diameter and geometric standard deviation were determined graphically using two-cycle logarithmic probit axes.
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: Mini-Nose Only Inhalation Chamber by ADG Developments LTD
- Exposure chamber volume: 6. 7 L
- Method of holding animals in test chamber: Polycarbonate holding tubes which seal to the chamber with an "O" ring during exposure.
- Source of air: Air compressor by JUN-AIR
- System of generating particulates/aerosols: Wright Dust Generator driven by a variable speed motor by Dayton
- Method of particle size determination: Eight-stage Andersen cascade impactor
TEST ATMOSPHERE (if not tabulated)
- Particle size distribution: An eight-stage Andersen cascade impactor was used to assess the particle size distribution of the test atmosphere. Samples were withdrawn from the breathing zone of the animals at two intervals. The filter paper collection stages were weighed before and after sampling to determine the mass collected upon each stage.
- MMAD (Mass median aerodynamic diameter) / GSD (Geometric st. dev.): The aerodynamic mass median diameter and geometric standard deviation were determined graphically using two-cycle logarithmic probit axes. See table 1 under "Any other information on materials and methods incl. tables" - Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 4 h
- Concentrations:
- See table 1 under "Any other information on materials and methods incl. tables"
- No. of animals per sex per dose:
- 5 males and 5 females per dose/ 3 doses
- Control animals:
- not specified
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of weighing: Prior to exposure and again on Days 7 and 14 or after death
- Frequency of observation: At least once daily for up to 14 days following exposure or until death occured
- Necropsy of survivors performed: yes
- Examinations performed: mortality, signs of toxicity, behavioral changes, body weight - Statistics:
- Mean + / - Standard Deviation
Results and discussion
- Preliminary study:
- See table 1 under "Any other information on materials and methods incl. tables"
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 0.054 - < 0.52 mg/L air
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Mortality:
- - At 2.02 mg/L exposure level, all animals died within 190 minutes of exposure to the test atmosphere
- At 0.52 mg/L exposure level, four male and four female rats died during exposure to the test atmosphere
- At 0.0542 mg/L exposure level, no mortality was observed - Clinical signs:
- other: - At 0.52 mg/L exposure level, the surviving animals were hypoactive and exhibited hunched posture, abnormal respiration and nasal discharge - At 0.0542 mg/L exposure level, all animals exhibited ocular and nasal discharge.
- Body weight:
- - At 0.52 mg/L exposure level, all surviving animals recovered by Day 3, gained body weight and appeared active and healthy for the remainder 14-day observation period.
- At 0.0542 mg/L exposure level, body weight was shown to increase over the observation period - Gross pathology:
- - At 2.02 mg/L exposure level, gross necropsy of the decedents revealed red, edematous lungs, normal trachea and clear wet nasal discharge.
- At 0.52 mg/L exposure level, gross necropsy of the decedents revealed red, edematous lungs, normal trachea and clear wet nasal discharge. No gross abnormalities were noted for the euthanized animal when necropsied at the conclusion of the 14-day observation period.
- At 0.0542 mg/L exposure level, no gross abnormalities were observed
Applicant's summary and conclusion
- Interpretation of results:
- Category 2 based on GHS criteria
- Conclusions:
- In conclusion, the acute inhalation toxicity of sodium azide, as determined in a study according to EPA guideline OPPTS 870.1300, lies between an LC50 (4 h) of 0.054 and 0.52 mg /L in male and female rats.
- Executive summary:
In an acute inhalation toxicity study according to EPA guideline OPPTS 870.1300, groups of young adult Sprague Dawley rats (5/sex) were exposed at test concentrations of 0.054, 0.52 and 2 mg/L via the inhalation route (nose only) to Sodium azide (>95% purity) in compressed air for 4 hours. After exposure, the animals were observed for a period of 14 days. At test concentration of 0.52 and 2.0 mg/L, mortality was observed. An increase in body weight was seen at 0.054 mg/L and gross pathology at test concentrations 0.52 and 2.0 mg/L revealed red, edematous lungs, normal trachea and clear wet nasal discharge. Thus, under the conditions of this study, the single exposure acute inhalation LC50 of the test substance is between 0.054 and 0.52 mg/L in male and female rats. Therefore classification as Acute Tox.2 (H330) according to CLP Regulation 1272/2008 is warranted.
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