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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
other: Authoritative data base
Title:
GCID : 166796
Author:
HAGEMAN,GJ,et al.
Year:
2011
Bibliographic source:
ACToR (Aggregated Computational Toxicology Resource):[HAGEMAN,GJ,et al.;BUTYLATED HYDROXYANISOLE,BUTYLATED HYDROXYTOLUENE&TERTBUTYLHYDROQUINONE ARE NOT MUTAGENIC IN THE SALMONELLA/MICROSOME ASSAY USING NEW TESTER STRAINS;MUTA.RES.208(3-4):207-211, 1988]

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other:
Principles of method if other than guideline:
Data is from ACTOR database
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
2-tert-butyl-4-methoxyphenol
EC Number:
204-442-7
EC Name:
2-tert-butyl-4-methoxyphenol
Cas Number:
121-00-6
Molecular formula:
C11H16O2
IUPAC Name:
2-tert-butyl-4-methoxyphenol
Details on test material:
- Name of test material (as cited in study report): tert-butyl-4-methoxyphenol
- Substance type: Organic
- Physical state: Solid

Method

Species / strainopen allclose all
Species / strain / cell type:
S. typhimurium TA 97
Additional strain / cell type characteristics:
not specified
Species / strain / cell type:
S. typhimurium TA 100
Additional strain / cell type characteristics:
not specified
Species / strain / cell type:
S. typhimurium TA 102
Additional strain / cell type characteristics:
not specified
Species / strain / cell type:
S. typhimurium, other: TA104
Additional strain / cell type characteristics:
not specified
Metabolic activation:
with and without
Metabolic activation system:
RAT, LIVER, S-9, AROCLOR
Test concentrations with justification for top dose:
1-1000 UG/PLATE
Vehicle / solvent:
DMSO
Details on test system and experimental conditions:
Test System : AMES SALMONELLA TYPHIMURIUM
Method : STANDARD PLATE

Results and discussion

Test resultsopen allclose all
Species / strain:
S. typhimurium TA 97
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Species / strain:
S. typhimurium TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Species / strain:
S. typhimurium TA 102
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Species / strain:
S. typhimurium, other: TA104
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

In an Ames test , tert-butyl-4-methoxyphenol, in dimethyl sulfoxide from doses 1-1000 ug/plate was not mutagenic in Salmonella typhimurium strains TA97, TA100,TA102 and TA104 with and without addition of S9 liver fractions from Aroclor induced rats.
Executive summary:

In an Ames test , tert-butyl-4-methoxyphenol, in dimethyl sulfoxide from doses 1-1000 ug/plate was not mutagenic in Salmonella typhimurium strains TA97, TA100,TA102 and TA104 with and without addition of S9 liver fractions from Aroclor induced rats.